Determination of staphylococcal enterotoxins in cheese by immunoenzyme assays
نویسندگان
چکیده
منابع مشابه
Staphylococcal Enterotoxins
Staphylococcus aureus (S. aureus) is a Gram positive bacterium that is carried by about one third of the general population and is responsible for common and serious diseases. These diseases include food poisoning and toxic shock syndrome, which are caused by exotoxins produced by S. aureus. Of the more than 20 Staphylococcal enterotoxins, SEA and SEB are the best characterized and are also reg...
متن کاملAmmonium sulfate coprecipitation antibody determination with purified staphylococcal enterotoxins.
The ammonium sulfate coprecipitation technique of Farr was applied in a study of the purified enterotoxins of Staphylococcus aureus. Ammonium sulfate coprecipitation of iodine-131-labeled enterotoxins A, B, and C, with the use of a 1.6 m concentration of (NH(4))(2)SO(4), revealed differences in the antigen-binding capacity of normal and immune rabbit sera for the enterotoxins. The coprecipitati...
متن کاملStaphylococcal enterotoxins in scarlet fever complicating chickenpox.
Two cases of scarlet fever are described, both following super-infection of chickenpox. Enterotoxin B and C producing staphylococci were the only pathogens identified. The role of staphylococcal and streptococcal toxins in the pathogenesis of scarlet fever and toxic shock syndrome is discussed.
متن کاملTwo common structural motifs for TCR recognition by staphylococcal enterotoxins
Superantigens are toxins produced by Staphylococcus aureus, called staphylococcal enterotoxins (abbreviated SEA to SEU). They can cross-link the T cell receptor (TCR) and major histocompatibility complex class II, triggering a massive T cell activation and hence disease. Due to high stability and toxicity, superantigens are potential agents of bioterrorism. Hence, antagonists may not only be us...
متن کاملDetermination of staphylococcal enterotoxin A in cheddar cheese produced without starter activity.
Three variants of the chloramine-T radioiodination method were used to iodinate staphylococcal enterotoxin A with 125I. Only one method consistently produced usable labels for radioimmunoassay. The iodine incorporation was 55 to 76%; the specific activity was 3.5 to 5.5 muCi/microgram of enterotoxin, and the label was extremely stable on storage at -20 degrees C. Determinations of the enterotox...
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ژورنال
عنوان ژورنال: Arhiv za bioloske nauke
سال: 2012
ISSN: 0354-4664,1821-4339
DOI: 10.2298/abs1204449j